|
KMID : 0364820200560010028
|
|
Korean Journal of Microbiology
2020 Volume.56 No. 1 p.28 ~ p.35
|
|
|
Antifungal activity of Bacillus subtilis isolates against toxigenic fungi
|
|
Hwang Ji-Seon
Song Hong-Gyu
|
|
|
Abstract
|
|
|
|
Toxigenic fungi can contaminate feed and grains and produce toxins which are toxic and carcinogenic to humans and animals at low concentration. This study aims to evaluate antifungal activity against toxigenic fungi by Bacillus subtilis AF11 and YR47 isolated from soil and their antifungal substances. These strains suppressed mycelial growth in the plate diffusion assay and showed the inhibition zones over 13.3 mm against 3 ochratoxigenic fugi and 4 fumonisinigenic fungi. AF11 and YR47 also inhibited sporulation of the target fungi by 77.7~ 100.0 and 75.4~100.0%, respectively compared to those of the control. They removed 38~90% of the fungal spores (10 7 spores/ml), and suppressed germination of the remaining spores by more than 94.9%. Ochratoxin A production decreased effectively when ochratoxigenic Aspergillus strains were cocultured with AF11 or YR47 in YES broth. It might be due to the antifungal substances including siderophore, β-1,3-glucanase, chitinase and protease produced by these strains. These results suggest that Bacillus subtilis AF11 and YR47 can be used to control growth of toxigenic fungi and their mycotoxin production.
|
|
|
KEYWORD
|
|
|
Bacillus subtilis, antifungal activity, antifungal substances, toxigenic fungi
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|